Jordon-Thaden , Ingrid Eastman , Facio Viccini, Lyderson , Buggs, Richard , Veruska C. Silva, Ana , Chamala, Srikar , Davenport, Ruth , Wu, Wei , Schnable, Patrick , Barbazuk, Brad , Soltis, Douglas , Soltis, Pamela .
Further exploration of genome evolution and gene expression in naturaland synthetic polyploids (Tragopogon, Asteraceae).
We present an integrated study of genome evolution, gene expression, and phenotype in two recently formed allotetraploid species (Tragopogon miscellus and T. mirus), their diploid parents (T. pratensis, T. dubius, and T. porrifolius),and three generations of synthetic allotetraploids (T. miscellus and T. mirus).We established a randomized block experiment for comparison of diploids and natural and synthetic allotetraploids. Using SNPs identified from 454 and Illumina sequencing of the diploids, we designed ~7000 possible SNP primers for Sequenom Mass-ARRAY analysis of gDNA and cDNA from ~500 individuals. Overall,700 SNPs for each set of parents and the respective allotetraploids were used(~1400 total). SNP variation between the diploid parents and is used to assess homeolog loss and changes in gene expression in the allotetraploids relative to their parents and to compare broadly sampled wild individuals with synthetics.We recorded phenotypic variation in many quantitative traits and will link these data to the gene loss/gene expression data. Phenotypes scored include growth rate, physiological robustness (measured in photosynthetic efficiency), mineral content, and reproductive timing and fitness.
Log in to add this item to your schedule
1 - University Of Florida, Biology, Bartram-Carr Hall, POBox 118525, Gainesville, FL, 32605, USA
2 - Universidade Federal De Juiz De Fora, Biologia, Instituto De Ciências Biológicas, Campus Da UFJF, Bairro Martelos, Juiz De Fora, N/A, 36036-900, Brazil
3 - Queen Mary University of London, School of Biological and Chemical Sciences, Mile End Road, London, E1 4NS, UK
4 - Embrapa Coastal Tablelands, Aracaju, 49025, Brazil
5 - University of Florida, Department of Biology, Gainesville, FL, 32611, USA
6 - Iowa State University, Center for Plant Genomics, Ames, IA, 50011, USA
7 - University of Florida, Dept. Biology, Gainsville, FL, 32611, USA
8 - University Of Florida, Florida Museum Of Natural History, PO BOX 117800, Gainesville, FL, 32611-7800, USA, 352/273-1964
Presentation Type: Oral Paper:Papers for Sections
Location: Delaware D/Hyatt
Date: Monday, July 9th, 2012
Time: 8:45 AM