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Abstract Detail


Recent Topics Posters

Ravirajan, Mangaiyarkarasi [1], laksmanan, Kannan [2].

Partial Purification and Characterization of Lectin from the Marine Plant Acanthophora Spicifera(Vahl.) Boergesen.

Haemagglutinins(lectins) are the compounds that are agglutinating (clumping) the blood cells. Lectins from various sources have been isolated, characterized and experimented in various preparative and analytical studies on biochemistry, cell biology and related areas. As the interest of application is growing rapidly, search of new sources for novel compounds as they are living in specilized habitats. But, not much work has been done in these largely available renewable natural resources, the Marine plants (seaweeds). The red seaweed, Acanthophoraspicifera (Vahl.)Boergesen, was collected from the Gulf of Mannar Biosphere Reserve,India that showed haemagglutinin activity in human blood (A, B, AB and O),chick blood, goat blood and sheep blood. The haemagglutinin activNTHOPHORAity of the crude (saline) extract was determined as 1:2, 1:4, 1:4 and 1:4 in human blood cells A, B, AB and O and1:4, 1:4 and 1:8 in chick, goat and sheep blood cells, respectively. The crude extract was purified by ammonium sulphate precipitation and Sephadex G-50 column chromatography. The purified lectin increased the activity to1:4, 1:4, 1:8 and 1:8 in human blood cells A, B, AB and O and 1:8, 1:8 and 1:16in chick, goat and sheep blood cells, respectively. In the different blood groups, the haemagglutinin activity of the extract of Acanthophora spicifera is44% to 100%.Minimum activity during the post monsoon season and the maximum activity during summer season. Chemical analysis revealed 13.96% of protein,5.64% of carbohydrate and 15.06% of total amino acids in purified lectin. The IR analysis revealed the functional groups. HPLC analysis revealed the presence of acidic, hydroxyl and basic amino acids. The lectin was functional in the pH range from 7 to 9.

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1 - 20-Indra Nagar, Kondur, Cuddalore, N/A, 607002, India
2 - CAS in Marine Biology, Chidambaram, TN, 608502, INDIA

Keywords:
Haemagglutinins (lectins)
seaweed
IR analysis
HPLC analysis.

Presentation Type: Recent Topics Poster
Session: P
Location: Battelle South/Convention Center
Date: Monday, July 9th, 2012
Time: 5:30 PM
Number: PRT008
Abstract ID:1301


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