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Abstract Detail


Recent Topics Posters

Borkowski, Daniel [1], McCleary, Tim [2], Romero-Severson, Jeanne [2].

Amplification of Introns within Stress Tolerance Candidate Genes in Northern red oak (Quercus rubra L.).

How individuals within a population respond to stressful conditions depends partially on the genotype of the individual. Many studies have identified candidate genes for stresses, but among the most important for terrestrial plant survival are genes which confer cold and drought tolerance. Furthermore, many genetic studies depend on housekeeping genes to account for evolution under conditions that would not differ among populations. We show here the successful amplification of segments of drought and freeze tolerance candidate genes and putative housekeeping genes in the species Northern red oak (Quercus rubra,L.). We designed primers to capture exon-intron-exon regions by utilizing cDNA sequences from the Fagaceae Genomics Project and the peach (Prunus persica) whole genome sequence. Probable gene annotations were determined using the Arabidopsis thaliana and Populus trichocarpa genome sequences. Annealing temperatures ranged between 55 °C and 68 °C for genes shown. We anticipate that these candidate gene sequences will allow us to investigate the genetic basis for the observed adaptive variation in oak taxa across their ranges.

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1 - University Of Notre Dame, Biological Sciences, 320 Galvin Life Science Bldg, Notre Dame, IN, 46556, USA
2 - University of Notre Dame, Biological Sciences, 320 Galvin Life Science Bldg, Notre Dame, IN, 46556, United States

Keywords:
nuclear genes
stress tolerance
Intron
Quercus.

Presentation Type: Recent Topics Poster
Session: P
Location: Battelle South/Convention Center
Date: Monday, July 9th, 2012
Time: 5:30 PM
Number: PRT028
Abstract ID:1321


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