Mayfield, Dustin , Edger, Patrick , Koch, Marcus , Pires, Joseph .
Phylogenomics and Placement of Polyploidy with Transcriptomes in Brassicaceae.
Cheaper sequencing costs due to next-generation sequencing (NGS) and corresponding computer tools have dramatically impacted the field of plant systematics. At this time, the most cost effective method for systematists allows multiplexing samples for sequencing of DNA. These resulting data provide researchers with the ability to obtain multiple perspectives on the evolutionary relationships among plants from three organellar genomes: the plastid (or chloroplast), mitochondrial, and nuclear genome. However, due to the lack of reads coming from nuclear genomes, it remains difficult to resolve relationships without more expensive, deep sequencing or targeted sequence capture. Here we show how transcriptomics can be leveraged for the identification of single copy nuclear genes, useful for phylogenomic inference.
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1 - University Of Missouri-Columbia, Division Of Biological Sciences, Life Science Center 311, 1201 East Rollins, Columbia, MO, 65211, USA
2 - University of Missouri - Columbia, Biological Sciences, 311 Life Science Center, 1201 E Rollins Rd, Columbia, MO, 65211, USA
3 - Universitat Heidelberg, COS, Biodiversity And Plant Systematics, Im Neuenheimer Feld 345, HEIDELBERG, N/A, D-69120, Germany
4 - University Of Missouri, 371 Bond Life Sciences Center, 1201 Rollins Street, Columbia, MO, 65211-7310, USA
next generation sequencing
Presentation Type: Oral Paper:Papers for Sections
Location: Delaware D/Hyatt
Date: Wednesday, July 11th, 2012
Time: 11:45 AM