Abstract Detail

Ecological Section

Piya, Sarbottam [1], Nepal, Madhav [2], Butler, Jack  [3], Larson, Gary [4], Neupane, Achal [5].

Population Genetics of Falcaria vulgaris (Sickleweed) in North America.

Falcaria vulgaris (sickleweed), a native to Europe and Western Asia,is an introduced plant in the United States. To date, introduction history and population genetics of US sickleweed populations have not been studied. Introduction and spread of the plant in the United States was reconstructed using natural history museum data and the population genetics of the plant was studied using RAPD (Randomly Amplified Polymorphic DNA) and microsatellite markers. Herbarium records show that the plant was first introduced no later than1922, probably independently along the East Coast and the Midwest of the United States. Since then, the plant has been documented in 37 counties in 15 states. Analysis using five polymorphic RAPD markers showed significant genetic variation among Midwest populations of sickleweed with the highest value in a population from the Fort Pierre National Grassland (% polymorphic loci= 70 and Nei’s gene diversity= 0.22). Microsatellite markers developed for Daucus carota were tested for their transferability to sickleweed. Screening of 47 microsatellite markers of Daucus resulted in the successful transferability of five markers to sickleweed. The genetic analysis using microsatellites is underway and the results of this analysis will be presented in the meeting. Results from the introduction history and genetic analyses of Falcaria vulgaris will provide valuable insight into the invasiveness of this plan in the United States and will be useful in devising appropriate control measures, and have an important implication in the study of other exotic invasive plants.

Broader Impacts:

Related Links:
Sickleweed genetics in Nepal Lab at South Dakota State university

1 -
2 - South Dakota State University, Biology And Microbiology, 245A Northern Plains Biostress Laboratory, Brookings, SD, 57007, USA
3 - USDA Forest Service, Rocky Mountains Research Station, 1730 Samco Road, Rapid City, SD, 57702, USA
4 - South Dakota State University, Department of Natural Resource Management, 322 Agricultural Hall, Brookings, Sd, 57007, USA
5 - South Dakota State University, Biology And Microbiology, 213 Northern Plains Biostress Laboratory, Brookings, SD, 57007, USA

Keywords:
Sickleweed
microsatellites
Falcaria vulgaris
population genetics.

Presentation Type: Oral Paper:Papers for Sections
Session: 21
Location: Union B/Hyatt
Date: Tuesday, July 10th, 2012
Time: 8:00 AM
Number: 21001
Abstract ID:274