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Abstract Detail


Conference Wide

Chester, Michael [1], Chanderbali, Andre [2], Soltis, Pamela [3], Soltis, Douglas [4].

Molecular cytogenetics: metaphase chromosome preparation, fluorescence in situ hybridization, and approaches for examining plant genomes.

In this workshop we will prepare metaphase chromosomes from root tips and set up a fluorescent in situ hybridization experiment. Attendees will be able to go through the procedure with roots from their own living plant collections or with materials supplied by the organizers. Day 1 will include a session of presentations in the afternoon covering a range of FISH/GISH applications, such as constructing karyotypes and studying genome-wide changes in diploid and polyploid lineages, integrating next-generation sequencing data into molecular cytogenetics, and using FISH to aid genome assemblies. Day 2 will provide opportunities to inspect slides and capture images from the FISH experiments.

Broader Impacts:


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1 - University Of Florida, Biology, Gainesville, FL, 32611, USA
2 - University Of Florida, 357 DICKINSON HALL MUSEUM ROAD & NEWELL DRIVE, P.O. BOX 117800, Gainesville, FL, 32611, USA, 352-392-1721 ex
3 - University Of Florida, Florida Museum Of Natural History, PO BOX 117800, Gainesville, FL, 32611-7800, USA, 352/273-1964
4 - University Of Florida, Department Of Botany, 220 BARTRAM HALL, Gainesville, FL, 32611, USA

Keywords:
FISH
GISH
BAC-FISH
next generation sequencing
genomics.

Presentation Type: Workshop
Session: WS01
Location: OSU/Hyatt
Date: Saturday, July 7th, 2012
Time: 8:00 AM
Number: WS01001
Abstract ID:30


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