Prabhakaran, Muthusamy , Veerasingam, Thennarasu , Duancan, Kaylia , Annamalai, Panneerselvam , Nooruddin, Thajuddin , Gunasekaran, M .
Separation of bioactive compounds, antifungal activity and enzyme production from typical and silver nanoparticle synthesis strain of Masoniella grisea.
Background: Fungi have proven to be a rich and promising source of novel antibacterial, antifungal, anticancer, antiplasmodial, anti-inflammatory and antiviral agents. Many of the fungi had been proven to be rich source of structurally novel and biologically active secondary metabolites, which are emerging as a significant new chemical resource for drug discovery. Recognizing the significance of fungi as a source of novel bioactive compounds, in the present study was deliberated. Methods: The antifungal activity of normal and silver nanoparticle synthesized Masoniella grisea was screened by using different solvent system such as pyridine, ethyl acetate and aqueous. The separation of bioactive compounds (Alkaloids, Phenols and Sterols) from typical and silver nanoparticle synthesis strain of Masoniella grisea using Thin Layer Chromatography and their antifungal activity were assayed. The production of cellulose and lipase enzyme was screened and the functional groups of isolated bioactive compounds were characterized by FT -IR and UV. Results: All the separated and purified bioactive compounds were exhibited very promising antifungal activity against all the fungal pathogens tested viz., Aspergillus flavus, A. terreus, A. luchensis, A. fumigatus and Candida albicans. The phenol compounds were showed better antifungal activity (inhibition zone range 25 - 30 mm) compared to alkaloids and sterol compounds. The functional groups were characterized by FT-IR and UV. The functional groups of purified phenols were showed as amide, I-III band, hydroxyl group, H - bonded, OH - stretch, O - NO2, Nitrates, Unsaturated nitrogen compounds, C=N stretch vibration, C-H bending, alkane N3 stretching vibrations azides, and hydrogen compounds respectively. Conclusions: The present study concluded that the silver nanoparticle synthesized Masoniella grisea strain was a potent source of antifungal agent and potent producer of cellulose and lipase enzyme compared with normal strain of Masoniella grisea.
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1 - Sri Pushpam College, Botany and Microbiology, Poondi, Tamilnadu, 613503, India
2 - Fisk University, Biology, 1000 17th Ave North, Nashville, TN, 37208, USA
3 - Sri Pushpam College, Botany and Microbiology, Poondi, Tamilnadu, 613503, USA
4 - Bharathidasan University, Microbiology, Tiruchirappalli, Tamilnadu, 620024, India
Presentation Type: Poster:Posters for Sections
Location: Battelle South/Convention Center
Date: Monday, July 9th, 2012
Time: 5:30 PM