Mercer, Elizabeth , Griffin, Brandi , Steele, Joshua , Goodrich, Katherine , Bush, Catherine .
The phylogeny of Asimina and Deeringothamnus (Annonaceae) using ISSR and morphological data.
The molecular phylogenetic classification of the North American genera Asimina and Deeringothamnus (Annonaceae) has never been determined with strong support and/or resolution. DNA sequences forthe two genera were shown in previous studies to be too similar to determine phylogenetic relationships. In this analysis, ISSR DNA fingerprinting techniques were used in order to elucidate the phylogeny of the group. DNA was extracted from accessions from both the field and herbarium. Six ISSR primers were used with15 species, including 11 of Asimina, two of Deeringothamnus, Annona glabra and Disepalum pulchrum. AAgarose gels stained with ethidium bromide were used to determine the presence and absence of bands. These data were analyzed in PAUP using Neighbor Joining algorithms. Morphological traits (including vegetative and floral scent characteristics) were included in addition to the molecular data. The preliminary conclusions strongly support the inclusion of Deeringothamnusin Asimina.
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1 - Valdosta State University, Biology, 1500 N. Patterson St. , Valdosta, GA, 31698, USA
2 - Widener University, Department Of Biological Sciences, Kirkbride Hall, 1 University Place, Chester, PA, 19013, USA
Presentation Type: Oral Paper:Papers for Sections
Location: Delaware B/Hyatt
Date: Wednesday, July 11th, 2012
Time: 4:00 PM