Growing the Next Generation in Plant Genomics
Egan, Ashley N. , Brewer, Michael , Doyle, Jeff .
Assessing the Polyploid origin of the genus Erythrina (Fabaceae).
Erythrina is a genus of about 150 tree species in the legume (Fabaceae) family, known for their strikingly beautiful, large flowered inflorescences. Several species are used horticulturally, planted all over the world as park and street trees. The genus is known to be polyploid, with 2n=42 chromosomes. We set out to investigate the origin of polyploidy in this genus through transcriptome characterization and comparison between E. crista-galli and E. herbacea. We compare distributions of synonymous substitutions among paralogs within each species as well as between orthologs among Erythrina species and Psophocarpus tetragonolobus, the winged bean, to assess the polyploid origin of the genus. By using divergence dating for sets of paralogs as well as orthologs, we can place a date of genome duplication relative to speciation between and within genera. We also characterized the three transcriptomes to determine transcriptome coverage and retention of duplicate genes post-polyploidy. The set of orthologous genes was also used to determine relative rates of substitution to investigate the impact of disparate rates of molecular evolution on dating polyploidy events.
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1 - East Carolina University, Biology, Howell Science Complex N303a, Mailstop 551, Greenville, NC, 27858, USA
2 - East Carolina University, Biology, Howell Science Complex N108, Greenville, NC, 27858, USA
3 - Cornell University, 412 Mann Library Building, ITHACA, NY, 14853-4301, USA
Presentation Type: Symposium or Colloquium Presentation
Location: Franklin C and D/Hyatt
Date: Monday, July 9th, 2012
Time: 3:30 PM