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Abstract Detail


Genomics / Proteomics

Lappin, Fiona [1], Brown, Jennifer [1], Weingartner, Laura [2], Hawkins, Kevin [1], McDermott, Ross [1], Harkess, Alex [3], Moore, Richard [1].

The investigation of selective sweeps within the Carica papaya X chromosome.

Carica papaya contains sex chromosomes that are evolutionary young; this allows for the investigation of the formation of and the evolutionary forces that affect early sex chromosomes. Previous investigations found four X/Y gene pairs within the sex determining region which displayed evidence of elevated levels of polymorphism within the Y and selective sweeps within the X reducing its diversity. Currently we have expanded this study to include over 20 X/Y gene pairs over the gene enriched areas of the X and Y, predicting that the previously observed patterns will be found across the X and Y and not restricted to the sex determining region. To further examine the patterns of selective sweeps across the X region, we intend to employ next generation sequencing to produce high coverage of a 2Mb targeted region within 12 female individuals of Carica papaya both within the X chromosome and autosomal regions. We propose to compare levels and patterns of diversity on the X chromosome to those on autosomes and assess what regions of the X harbour patterns of diversity consistent with a selective sweep, as well as determine the extent of that signature. It is the goal of this investigation to assess the levels of selective sweeps across the X chromosome and to identify possible targets of those sweeps.

Broader Impacts:


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1 - Miami University, Botany, 316 Pearson Hall, Oxford, OH, 45056, USA
2 - Indiana University, Jordan Hall, 1001 E Third St., Bloomington, IN, 47405, USA
3 -

Keywords:
Carica papaya
Selective sweep
Sex chromosome.

Presentation Type: Poster:Posters for Topics
Session: P
Location: Battelle South/Convention Center
Date: Monday, July 9th, 2012
Time: 5:30 PM
Number: PGP002
Abstract ID:455


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