Heckathorn, Scott , Mishra, Sasmita .
Monitoring nutrient stress in plants using protein biomarkers and ELISA.
Mineral nutrient stress is widespread in crops, and the negative economic impact of this is substantial. There is a pressing need to develop additional methods to detect incipient nutrient stress to mitigate crop damage, identify specific nutrient stressors, or select desirable "nutrient" genotypes. We are developing ELISA-based diagnostic assays for several commonly-problematic nutrients. A bioinformatics approach is used to identify appropriate conserved domains in specific key nutrient metabolism proteins, and then short peptides of these domains are used to generate protein-specific polyclonal antibodies. Antibodies are antigen-purified if needed and checked for specificity. ELISA assays are calibrated with antigen, optimized, and their sensitivity determined using plants grown over a range of nutrient levels. We have generated ELISAs for transport proteins for B (BOR1, NIP5), P (Pht1), and Fe (FRO1), and are currently developing assays to N, Ca, and Mg metabolic proteins. These ELISA-based assays will supplement other available tools for detecting and evaluating nutrient stress or identifying desirable genotypes, and in some ways will offer distinct advantages over current methods.
Log in to add this item to your schedule
1 - University of Toledo, Environmental Sciences, MS 604, 2801 W. Bancroft St., Toledo, OH, 43606, USA
Presentation Type: Poster:Posters for Sections
Location: Battelle South/Convention Center
Date: Monday, July 9th, 2012
Time: 5:30 PM