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Abstract Detail


Physiological Section

Heckathorn, Scott [1], Mishra, Sasmita [1].

Monitoring nutrient stress in plants using protein biomarkers and ELISA.

Mineral nutrient stress is widespread in crops, and the negative economic impact of this is substantial. There is a pressing need to develop additional methods to detect incipient nutrient stress to mitigate crop damage, identify specific nutrient stressors, or select desirable "nutrient" genotypes. We are developing ELISA-based diagnostic assays for several commonly-problematic nutrients. A bioinformatics approach is used to identify appropriate conserved domains in specific key nutrient metabolism proteins, and then short peptides of these domains are used to generate protein-specific polyclonal antibodies. Antibodies are antigen-purified if needed and checked for specificity. ELISA assays are calibrated with antigen, optimized, and their sensitivity determined using plants grown over a range of nutrient levels. We have generated ELISAs for transport proteins for B (BOR1, NIP5), P (Pht1), and Fe (FRO1), and are currently developing assays to N, Ca, and Mg metabolic proteins. These ELISA-based assays will supplement other available tools for detecting and evaluating nutrient stress or identifying desirable genotypes, and in some ways will offer distinct advantages over current methods.

Broader Impacts:


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1 - University of Toledo, Environmental Sciences, MS 604, 2801 W. Bancroft St., Toledo, OH, 43606, USA

Keywords:
nutrients
biomarkers
transport proteins.

Presentation Type: Poster:Posters for Sections
Session: P
Location: Battelle South/Convention Center
Date: Monday, July 9th, 2012
Time: 5:30 PM
Number: PPS005
Abstract ID:462


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