Mishra, Sasmita , Heckathorn, Scott , Frantz, Jonathan , Gray, John .
The effects of boron, light, CO2, and N on levels of boron-transport proteins, BOR1 and NIP5;1, indicate transporter-specific responses to both B and other plant resources.
Boron (B) is an essential micronutrient required for plant growth and development. Recently, two major B-transporter proteins, BOR1 and NIP5;1, have been identified and partially characterized. BOR1 is a high-affinity B transporter involved in xylem loading, and NIP5;1 acts as a channel protein for B-uptake from soil. To date, studies of these B transporters have investigated their (mostly mRNA) expression only in response to variation in B availability, and the influence of other factors, such as those affecting plant resource status, have not been studied. To address this, we have grown several plant species under ambient or elevated CO2, different non-photoinhibitory light levels, and low and high N, while also manipulating B availability. Expression levels of BOR1 and NIP5;1 were measured by Western blotting or ELISA, with protein-specific antibodies that we developed using bioinformatics. Surprisingly, BOR1 level was affected little by B availability, but increased with light, CO2, and N. In contrast, NIP5;1 increased significantly with both B and the other factors. Our results indicate that the major plant B transporters respond to both B availability, as well as environmental factors that affect plant resource status, but these transporters respond differently, and they indicate that NIP5;1 is the main "transporter response" to variation in B availability.
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1 - University of Toledo, Environmental Sciences, MS 604, 2801 W. Bancroft St., Toledo, OH, 43606, USA
2 - University of Toledo, U.S. Department of Agriculture-Agricultural Research , 2801 W Bancroft St., Toledo, Ohio, 43606, USA
3 - University of Toledo, Biological Sc, 2801 W Bancroft. St, Toledo, Ohio, 43606, USA
Presentation Type: Poster:Posters for Sections
Location: Battelle South/Convention Center
Date: Monday, July 9th, 2012
Time: 5:30 PM