Developmental and Structural Section
Bhawana, Bhawana , Miller, Joyce , Cahoon, Aubrey .
Ultra-structural Anatomy of Immature Plastids Using TEM and FIB-SEM Electron Microscopy.
Plastids are a group of organelles present in the cells of higher and lower plants and algae. Along with photosynthesis, they provide a variety of biochemical capabilities and are one of the defining features of plant cells. We are interested in studying the often overlooked pro-plastid (the progenitor of all plastid types). One of our approaches to gaining a better understanding of this organelle is to employ electron tomography to make 3-Dimensional renderings of the cellular and tissue environments where pro-plastids reside. We are using Arabidopsis seeds, Arabidopsis leaf and root calli as our initial model tissues. So far these tissues have been successfully fixed, embedded, and sectioned for TEM in preparation for tomographic analysis. A second approach, Focused Ion Beam-SEM (FIB-SEM), is also underway. FIB-SEM uses an ion beam to generate serial sections followed by 3D reconstruction of the target material. TEM and FIB-SEM micrographs of Arabidopsis seeds and callus have been generated and will be presented.
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1 - Middle Tennessee State University, Molecular Biosciences, 1301 East Main Street , Murfreesboro, Tennessee, 37132, USA
2 - Middle Tennessee State University, MTSU Interdisciplinary Microanalysis and Imaging Center , 1301 East Main Street, Murfreesboro, Tennessee, 37132, USA
3 - Biology, 1301 E. Main St, Box 60, Murfreesboro, TN, 37132, USA
Presentation Type: Poster:Posters for Sections
Location: Battelle South/Convention Center
Date: Monday, July 9th, 2012
Time: 5:30 PM