McDaniel, James , Moore, DeAnne , Gannicott, Priscilla , Cowden, Nancy .
Does the Polyploid Derivative Smell Just as Sweet: Rosa carolina and R. palustris.
Based on cytological and genealogical evidence, Rosa palustris and R. carolina, members of the R. carolina complex (section Cinnamomeae), share a progenitor-derivative relationship whereby hybridization of R. palustris with another, eastern North American, diploid species gave rise to the tetraploid, R. carolina. Subtle morphological differences can make accurate field identification of R. carolina and R. palustris challenging, but the species tend to prefer distinct habitats. In Bedford Co., Virginia, USA, we encountered a formerly cultivated, agricultural field within the flood plain of the Big Otter River that provides a heterogeneous environment supporting sympatric populations with overlapping flowering times. Our particular interest focuses on the comparative characterization of the floral fragrances and insect visitors of these roses, in an effort to understand events leading to the origins of R. carolina. During late May-June 2010, 2011 and 2012, we investigated the fragrance composition and pollinators of R. palustris and R. carolina. Fragrance samples were collected via static headspace, solid-phase microextraction (SPME). A Thermo electron-focus GC/DSQ MS and XCalibur 1.4 software generated total ion chromatograms, and mass spectra were obtained and analyzed using NIST MS Search 2.0 to identify the compounds collected. Insect collections, accomplished on the same days as floral fragrance sampling and associated with flowers specifically sampled, were identified and are maintained as voucher specimens at Lynchburg College. Ultimately, characterizing fragrance signatures and identifying pollinators provides the opportunity to determine whether R. carolina and R. palustris share more than a set of chromosomes.
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1 - Lynchburg College, Biology, 1501 Lakeside Drive, Lynchburg, VA, 24501, USA
2 - TriTech Laboratories, Lynchburg, VA, 24501, USA
3 - Lynchburg College, Chemistry, 1501 Lakeside Drive, Lynchburg, VA, 24501, USA
Presentation Type: Poster:Posters for Sections
Location: Battelle South/Convention Center
Date: Monday, July 9th, 2012
Time: 5:45 PM