Growing the Next Generation in Plant Genomics
Holliday, Jason , Zhou, Lecong .
Toward genome-wide association mapping and landscape genomics in a model tree: capture and re-sequencing of the black cottonwood exome in a highly diverse, rangewide sample.
Most ecologically and economically important tree species in the United States have broad geographic ranges, and local adaptation is therefore a key determinant of forest productivity and ecosystem health. With climate change substantially altering adaptive landscapes, there is an increasing misalignment between genotypes and phenotypes that impacts the long-term survival and distribution of these species. We are taking a population genomics approach to dissect the genomic basis for local adaptation in Populus trichocarpa (black cottonwood). Sequence capture technology is being employed to retrieve exons, upstream regulatory regions, and intergenic control regions for all genes in a population of P. trichocarpa that encompasses most of the latitudinal and altitudinal range of the species. To date, this approach has been tested on 48 P. trichocarpa genotypes, and will be extended to an additional 450 genotypes. Data from the preliminary capture and sequencing will be discussed, including capture efficiency and associate design considerations, population structure, and other demographic processes that are at play in this widespread, wind-pollinated, obligate outcrossing species. Ultimately, the goal of this study is to both begin to answer long standing questions in evolutionary ecology about the genomic architecture of adaptation, as well as to provide ecologically-relevant genetic markers to poplar breeders who may wish to exploit naturally occurring genetic variation for tree improvement in a changing climate.
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1 - Virginia Tech, Forest Resources and Environmental Conservation, 304 Cheatham Hall, Blacksburg, VA, 24061, USA
Presentation Type: Symposium or Colloquium Presentation
Location: Franklin C and D/Hyatt
Date: Monday, July 9th, 2012
Time: 1:30 PM