Liu, Xiang , Xie, Deyu , Feng, Chunmiao , Qu, Rongda , Franks, Robert , Xiang, Qiuyun (Jenny) .
Agrobacterium-mediated genetic transformation in Cornus canadensis - a model for investigating plant genetics and development in dogwoods (Cornaceae).
Cornus canadensisis a rhizomatous perennial, producing annual floral stems above the ground. To faciliate functional analysis of genes involved in the inflorescence development and evolution in the genus, a genetic transformation system is required. Based on an efficient regeneration protocol we previously developed for C. canadensis, we aimed to develop and optimize a genetic transformation method in the species. Using the Green Fluorescent Protein (GFP) gene as a reporter and hygromycin phosphotransferase (Hpt) gene as the selection marker, we successfully developed an Agrobacterium-mediated transformation system in C. canadensis and obtained trangenic plants.The protocol has allowed us to begin testing functions of genes homologuous to key regulators in Arabidopsis floral and inflorecence development (e.g., AP3, TFL1, LFY, AP1, FT). To our knowledge, no genetic transformation of dogwood species has been reported. This system paves the way to establish C. canadnesis as a new model for evolutionary developmental genetics of plants closely related to the dogwood family.
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1 - North Carolina State University, Department of Plant Biology, Campus Box 7612, Raleigh, North Carolina, 27695, United States
2 - North Carolina State University, Department of Crop Sciences, Raleigh, North Carolina, 27695, United States
3 - North Carolina State University, Department of Genetics, Raleigh, North Carolina, 27695, United States
Agrobacterium mediated - genetic transformation
Presentation Type: Oral Paper:Papers for Sections
Location: Delaware D/Hyatt
Date: Monday, July 9th, 2012
Time: 9:45 AM