Abstract Detail

Principles of crucifer evolution

Dewoody, Jennifer [1], McKay, John [2].

Conservation and diversity in drought response across the Brassicaceae.

As the first plant genome to be sequenced, the Arabidopsis thaliana genome is a fundamental reference for plant comparative genomics. An emerging question in agriculture is the degree to which breeding will be informed by results from the model plant Arabidopsis. A logical starting point for this endeavor is in the Brassica oilseed crops. We applied comparative transcriptomics to examine differences in coding sequence and gene expression in response to drought in two major genera in the Brassicaceae: Brassica and Arabidopsis. Sequence polymorphism and divergence were analyzed for a set of genes previously shown to act in the water-deficit signaling pathway. In addition,gene expression regulation in response to drought was compared between A.thaliana and B. napus. Using the abundant genomic data available for A. thaliana,predicted gene ontologies were extracted for those genes differentially regulated in response to drought, providing a preliminary network analysis that can be compared among species. Given the critical role adaptation to water deficit plays in plant evolution, these analyses harness the power of NGS data to provide insight into adaptive sequence divergence and diversification among Crucifers.

Broader Impacts:

1 - Colorado State University, Bioagricultural Sciences And Pest Management, C129 Plant Sciences, Fort Collins, CO, 80523-1177, United Kingdom
2 - Colorado State University, Bioagricultural Sciences and Pest Management, C129 Plant Sciences, Fort Collins, CO, 80523-1177, USA

Keywords:
RNA-sequencing
Arabidopsis
Brassica
drought.

Presentation Type: Symposium or Colloquium Presentation
Session: C4
Location: Delaware C/Hyatt
Date: Tuesday, July 10th, 2012
Time: 4:45 PM
Number: C4013
Abstract ID:762