Zimmer, Elizabeth , Johnson, Gabriel , Nagi, Suzanne , Wollaeger, Heidi , Figlar, Richard .
Genetic variability in Magnolia acuminata (L.) populations in the United States.
Magnolia acuminata (cucumber tree) occurs as scattered individuals in deciduous forests of the eastern United States and southern Ontario. Using morphological characters, Hardin (1954) proposed that there were three fairly geographically distinct varieties of the species, M.acuminata var. cordata, M. acuminata L. var. acuminata and M. acuminata var. ozarkensis, distributed in an eastern to western manner. Leaf tissue from individual trees at twenty sites across the range of the species was collected for DNA extractions. Four chloroplast DNA regions (trnH-psbA,ndhF-rpl32, atpF and psbK) were amplified and sequenced; no variation for any of the markers was detected in the over eighty individuals sampled. In order to access more rapidly evolving microsatellite-containing regions of the DNA, thirty primer pairs, developed for M.stellata, an Asian species also in the section Yulania, were tested for amplification and polymorphism in M. acuminata. Elevenprimer pairs were chosen and profiles for all M. acuminata samples were generated. The number of alleles detected per primer pair ranged from four to fourteen. The variation observed was analyzed both via Principal Component Analysis (PCA) and in the program STRUCTURE. There were three populations with fairly distinct patterns, one of which represented a western locality. Overall, however, there was overlap of most profiles across the geographic range sampled.
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1 - Smithsonian National Museum of Natural Histor, Botany, Museum Support Center, 4210 Silver Hill Rd., Suitland, MD, 20746, USA
2 - Smithsonian National Museum of Natural Histor, Botany/LAB, Museum Support Center, 4210 Silver Hill Rd., Suitland, MD, 22304, USA
3 - Smithsonian National Museum of Natural History, Botany, Museum Support Center, 4210 Silver Hill Rd., Suitland, MD, 20746, USA
4 - Michigan State University, Horticulture, Plant and Soil Sciences Building, East Lansing, MI, 48824-1325, USA
5 - Magnolia Society, Newton Rd., Pickens, SC, 29671, USA
Presentation Type: Poster:Posters for Sections
Location: Battelle South/Convention Center
Date: Monday, July 9th, 2012
Time: 5:30 PM