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Abstract Detail


Pteridological Section/AFS

Grusz, Amanda [1], Windham, Michael [2], Pryer, Kathleen [3].

Using next generation sequencing to develop microsatellite markers in ferns.

Microsatellites are rapidly evolving, co-dominant molecular markers that have been used in plant studies of genetic variation since the early 1980s. The time and expense involved in developing these markers, however, has limited their widespread use, particularly for studying non-model organisms. Although the advent of next generation sequencing technology has dramatically eased these constraints, fern biologists have yet to embrace it for developing microsatellite markers. Here, we use the autotriploid fern Cheilanthes lindheimeri as a case study to provide an explicit guide on how and why to develop fern microsatellite markers from next generation sequencing data. The accessibility of these markers has the same exciting potential as did isozymes 30 years ago to answer questions that have long-plagued fern evolutionary and population biology.

Broader Impacts:


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1 - Duke University, Box 90338, Durham, NC, 27708, USA
2 - DUKE UNIVERSITY, Department Of Biology, BOX 90338, DURHAM, NC, 27708, USA
3 - Duke University, Science Drive, Durham, NC, 27708-0338, USA

Keywords:
next generation sequencing
microsatellites
SSR markers
cheilanthoid ferns
genotyping.

Presentation Type: Oral Paper:Papers for Sections
Session: 11
Location: Union E/Hyatt
Date: Monday, July 9th, 2012
Time: 1:30 PM
Number: 11001
Abstract ID:946


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