Grusz, Amanda , Windham, Michael , Pryer, Kathleen .
Using next generation sequencing to develop microsatellite markers in ferns.
Microsatellites are rapidly evolving, co-dominant molecular markers that have been used in plant studies of genetic variation since the early 1980s. The time and expense involved in developing these markers, however, has limited their widespread use, particularly for studying non-model organisms. Although the advent of next generation sequencing technology has dramatically eased these constraints, fern biologists have yet to embrace it for developing microsatellite markers. Here, we use the autotriploid fern Cheilanthes lindheimeri as a case study to provide an explicit guide on how and why to develop fern microsatellite markers from next generation sequencing data. The accessibility of these markers has the same exciting potential as did isozymes 30 years ago to answer questions that have long-plagued fern evolutionary and population biology.
Log in to add this item to your schedule
1 - Duke University, Box 90338, Durham, NC, 27708, USA
2 - DUKE UNIVERSITY, Department Of Biology, BOX 90338, DURHAM, NC, 27708, USA
3 - Duke University, Science Drive, Durham, NC, 27708-0338, USA
next generation sequencing
Presentation Type: Oral Paper:Papers for Sections
Location: Union E/Hyatt
Date: Monday, July 9th, 2012
Time: 1:30 PM