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Abstract Detail


Genomics / Proteomics

Der, Joshua [1], Ayyampalayam, Saravanaraj [2], Burnett, James [3], Chamala, Srikar [4], Chanderbali, Andre [4], Estill, James [2], Jiao, Yuannian [1], Liu, Kun [3], Ralph, Paula [1], Lan, Tianying [5], Lyons, Eric [6], Tang, Haibao [7], Tomsho, Lynn P. [8], Wafula, Eric [1], Walts, Brandon [4], Albert, Victor [5], Barbazuk, Brad [4], Ma, Hong [1], Leebens-Mack, Jim [2], Palmer, Jeffrey [9], Rounsley, Steve [10], Sankoff, David [11], Schuster, Stephan C. [8], Soltis, Douglas [4], Soltis, Pamela [12], Wessler, Susan [3], Wing, Rod [13], dePamphilis, Claude [1].

The draft genome sequence of Amborella trichopoda sheds light on the ancestral angiosperm genome.

The origin and early diversification of flowering plants (angiosperms) had profound impacts on Earth's biota, providing the raw genetic material from which most crops and economically important plants were derived. The diversification of genes, genomes, and important traits cannot be adequately interpreted without a comparative evolutionary framework firmly rooted with genome sequences from basal angiosperms. As the sister species to all other extant flowering plants, Amborella trichopoda holds a singular position in the flowering plant tree of life for establishing this comparative genomics framework. Toward this goal, we have sequenced, assembled, and annotated the nuclear genome sequence of Amborella trichopoda using a combination of multi-platform whole genome shotgun sequencing, Fluorescent In Situ Hybridization (FISH), and genome-wide optical mapping. I will present an overview of the genome assembly and annotation and provide a look at the evolutionary insights enabled by this remarkable resource. The Amborella Genome Sequencing Project is a collaborative NSF-funded project that seeks to produce a high-quality finished genome sequence that will serve as a reference in comparative evolutionary analyses to shed light on the genomic characteristics of the last common ancestor of extant flowering plants. Data are publicly available through the Amborella Genome Database, hosted at http://amborella.org.

Broader Impacts:


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Related Links:
Amborella Genome Database


1 - Penn State University, Dept. Biology, 101 Life Science Bldg, University Park, PA, 16802, USA
2 - University of Georgia, Dept. Plant Biology, 4504 Miller Plant Sciences, Athens, GA, 30602, USA
3 - University of California, Riverside, Dept. Botany and Plant Sciences, Riverside, CA, USA
4 - University of Florida, Dept. Biology, Gainsville, FL, 32611, USA
5 - University at Buffalo, Biological Sciences, 109 Cooke Hall, Buffalo, NY, 14260, USA
6 - University of Arizona, iPlant Collaborative, Tucson, AZ, 85721, USA
7 - J. Craig Venter Institute, Rockland, MD, USA
8 - Penn State University, Dept. Biochemistry and Molecular Biology, University Park, PA, 16802, USA
9 - Indiana University, Department Of Biology, Jordan Hall, 1001 East Third Street, Bloomington, IN, 47405, USA
10 - Dow Agrosciences, 9330 Zionville Road, Indianapolis, IN, 46033, USA
11 - University of Ottawa, Department of Mathematics and Statistics, Ottawa, ON, CANADA
12 - University Of Florida, Florida Museum Of Natural History, PO BOX 117800, Gainesville, FL, 32611-7800, USA, 352/273-1964
13 - University of Arizona, School of Plant Sciences, 1657 E. Helen St., Tucson, AZ, 85721, USA

Keywords:
Amborella
Amborella trichopoda
Whole Genome Shotgun
Genome Assembly
Genome evolution
Ancestral Genome
Nuclear Genome
FISH
Optical Map
angiosperm
Basal Angiosperm.

Presentation Type: Oral Paper:Papers for Topics
Session: 44
Location: Union B/Hyatt
Date: Wednesday, July 11th, 2012
Time: 9:00 AM
Number: 44005
Abstract ID:952


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